Analysis for the Combination Expression of CK20, FABP1 and MUC2 is Sensitive for the Prediction of Peritoneal Recurrence in Gastric Cancer

• Published in: Japanese journal of clinical oncology Vol. 42; no. 2; pp. 148 - 152
• Main Authors: Satoh, Yumiko, Mori, Kazuhiko, Kitano, Kentaro, Kitayama, Joji, Yokota, Hiromitsu, Sasaki, Hiroki, Uozaki, Hiroshi, Fukayama, Masashi, Seto, Yasuhiro, Nagawa, Hirokazu, Yatomi, Yutaka, Takai, Daiya
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2012
• Subjects: Antineoplastic Combined Chemotherapy Protocols - therapeutic use; Carcinoembryonic Antigen - metabolism; Disease-Free Survival; Fatty Acid-Binding Proteins - genetics; Fatty Acid-Binding Proteins - metabolism; Gene Expression Regulation, Neoplastic; Humans; Kaplan-Meier Estimate; Keratin-20 - genetics; Keratin-20 - metabolism; Mucin-2 - genetics; Mucin-2 - metabolism; Neoplasm Invasiveness; Peptides - metabolism; Peritoneal Neoplasms - metabolism; Peritoneal Neoplasms - mortality; Peritoneal Neoplasms - prevention & control; Peritoneal Neoplasms - secondary; Predictive Value of Tests; Recurrence; Reverse Transcriptase Polymerase Chain Reaction; Risk Assessment; Risk Factors; RNA, Messenger - metabolism; Sensitivity and Specificity; Stomach Neoplasms - drug therapy; Stomach Neoplasms - metabolism; Stomach Neoplasms - mortality; Stomach Neoplasms - pathology; Trefoil Factor-1; Trefoil Factor-2; Tumor Suppressor Proteins - metabolism; peritoneal recurrence; gastric cancer
• ISSN: 0368-2811; 1465-3621
• DOI: 10.1093/jjco/hyr179

Prediction of peritoneal recurrence in gastric cancer patients is important for application of adjuvant chemotherapy. After surgery, occasional patients have peritoneal recurrence despite negative cytology of the peritoneal washings. Thus, molecular detection of a subliminal number of cancer cells in peritoneal washings may overcome the sensitivity limitation of conventional cytology. In this study, expressions of five specific marker genes, namely, TFF1, TFF2, CK20, FABP1 and MUC2, were evaluated for their usefulness as markers of micro-dissemination. It was found that reverse transcriptase-polymerase chain reaction for these five genes yielded results highly specific for the depth of invasion and disease stage. Furthermore, the expression of CK20, FABP1 and MUC2 was a reliable prognostic indicator of peritoneal metastasis. Our results suggest that evaluation of the expression of CK20, FABP1 and MUC2 in peritoneal washings is a useful tool for identifying patients at high risk of peritoneal recurrence who may need adjuvant chemotherapy.