Brevibacillus brevis HNCS-1: a biocontrol bacterium against tea plant diseases

• Published in: Frontiers in microbiology Vol. 14; p. 1198747
• Main Authors: Yang, Wenbo, Yang, Hui, Bao, Xiaocun, Hussain, Mehboob, Bao, Qiang, Zeng, Zexuan, Xiao, Chun, Zhou, Lingyun, Qin, Xiaoping
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 13.09.2023
• Subjects: antagonistic activity; antimicrobial peptides; Brevibacillus brevis; edeine; genome annotation; Microbiology; pan-genome analysis
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1198747

As a biocontrol bacteria, Brevibacillus has been the subject of extensive research for agricultural applications. Antibacterial peptides (AMPs) are the main antibacterial products of Brevibacillus . This study isolated a strain of Br. brevis HNCS-1 from tea garden soil, and the strain has an antagonistic effect against five types of pathogens of tea diseases, namely Gloeosporium theae-sinensis, Elsinoe leucospira, Phyllosticta theaefolia, Fusarium sp., and Cercospora theae . To determine the genetic characteristics implicated in the biocontrol mechanism, the genome sequence of the HNCS-1 strain was obtained and analyzed further, and the data are deposited in the GenBank repository (No. CP128411 ). Comparative genomics analyses revealed that the HNCS-1 strain and 17 public Br. brevis share a core genome composed of 3,742 genes. Interestingly, only one non-ribosomal peptide synthetase (NRPS) gene cluster annotated as edeine is present in the core genome. And UHPLC-MS/MS detection results showd that edeine B and edeine A were the principal antibacterial peptides in the HNCS-1 strain. This study proves that edeine is the main antibacterial peptide of Br. brevis , and provides a new strategy for the identification of antibacterial products from other biocontrol bacteria.