Genetic diversity of bovine ulcerative mammary dermatitis-associated Treponema

• Published in: Veterinary microbiology Vol. 136; no. 1; pp. 192 - 196
• Main Authors: Stamm, L.V., Walker, R.L., Read, D.H.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 14.04.2009; Amsterdam; New York: Elsevier; Elsevier
• Subjects: 16S-23S rDNA intergenic spacer region; Animals; bacterial infections; Bacteriology; Base Sequence; Biological and medical sciences; Biopsy - veterinary; Bovine udder lesions; Breast Diseases - microbiology; Breast Diseases - veterinary; Cattle; cattle diseases; Cattle Diseases - microbiology; dermatitis; Dermatitis - microbiology; Dermatitis - veterinary; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; epidemiological studies; etiology; Female; Fundamental and applied biological sciences. Psychology; Genetic Variation; intergenic spacer region; mammary gland diseases; microbial genetics; Microbiology; Miscellaneous; molecular epidemiology; Molecular Sequence Data; nucleotide sequences; Papillomatous digital dermatitis; pathogen identification; Phylogeny; Polymerase Chain Reaction - veterinary; ribosomal DNA; risk assessment; Sequence Alignment; sequence analysis; skin lesions; strain differences; Treponema; Treponema - genetics; Treponemal Infections - microbiology; Treponemal Infections - veterinary; udders; Ulcerative mammary dermatitis; Ulcerative mammary dermatitis; 16S-23S rDNA intergenic spacer region; Bovine udder lesions; Papillomatous digital dermatitis; Treponema; Skin disease; Spirochaetales; Bovine; Microbiology; Ribosomal DNA; Genetic diversity; Dermatitis; Vertebrata; Mammalia; Bacteria; Artiodactyla; Lesion; Treponemataceae; Ulceration; Veterinary; Ungulata
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2008.10.022

The etiology of bovine ulcerative mammary dermatitis (UMD) is poorly characterized. The goal of this study was to genetically analyze spirochetes present in UMD lesions. DNA prepared from UMD lesion biopsies and from spirochetes cultured from the corresponding lesion biopsies was PCR amplified using primers for the 16S rDNA-tRNA(ile) intergenic spacer region (ISR) of Treponema 16S-23S rDNA. Analysis of cloned ISR amplicons from three cultivable UMD-associated spirochetes indicated that two isolates cluster closely with cultivable papillomatous digital dermatitis (PDD)-associated and human-associated Treponema phylotypes, while the remaining isolate is unique. Analysis of ISR amplicons from UMD lesion biopsies identified additional not-yet-cultivable Treponema phylotypes. Our results revealed the presence of a genetically diverse Treponema population in an UMD lesion.