The Resistance Responses of Potato Plants to Potato Virus Y Are Associated with an Increased Cellular Methionine Content and an Altered SAM:SAH Methylation Index

• Published in: Viruses Vol. 13; no. 6; p. 955
• Main Authors: Spechenkova, Nadezhda, Fesenko, Igor A., Mamaeva, Anna, Suprunova, Tatyana P., Kalinina, Natalia O., Love, Andrew J., Taliansky, Michael
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 21.05.2021; MDPI
• Subjects: Biosynthesis; Climate change; Cultivars; Disease resistance; Enzymes; Flowers & plants; Gene expression; Heat resistance; High temperature; Infections; isobaric tags for relative and absolute quantitation (iTRAQ); Metabolism; Metabolites; Methionine; methionine cycle; Peptides; plant virus resistance; Polyamines; potato virus Y; Potatoes; Proteins; Proteomics; Reagents; Transcription; Viruses; United States > US; Chicago Illinois
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13060955

Plant-virus interactions are frequently influenced by elevated temperature, which often increases susceptibility to a virus, a scenario described for potato cultivar Chicago infected with potato virus Y (PVY). In contrast, other potato cultivars such as Gala may have similar resistances to PVY at both normal (22 °C) and high (28 °C) temperatures. To elucidate the mechanisms of temperature-independent antivirus resistance in potato, we analysed responses of Gala plants to PVY at different temperatures using proteomic, transcriptional and metabolic approaches. Here we show that in Gala, PVY infection generally upregulates the accumulation of major enzymes associated with the methionine cycle (MTC) independently of temperature, but that temperature (22 °C or 28 °C) may finely regulate what classes accumulate. The different sets of MTC-related enzymes that are up-regulated at 22 °C or 28 °C likely account for the significantly increased accumulation of S-adenosyl methionine (SAM), a key component of MTC which acts as a universal methyl donor in methylation reactions. In contrast to this, we found that in cultivar Chicago, SAM levels were significantly reduced which correlated with the enhanced susceptibility to PVY at high temperature. Collectively, these data suggest that MTC and its major transmethylation function determines resistance or susceptibility to PVY.