Synthesis of Epstein-Barr Virus after Activation of the Viral Genome in a "Virus-Negative" Human Lymphoblastoid Cell (Raji) Made Resistant to 5-bromodeoxyuridine

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 69; no. 1; pp. 78 - 82
• Main Authors: Hampar, Berge, Derge, Jeffery G., Martos, Lidia M., Walker, John L.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01.01.1972; National Acad Sciences
• Subjects: Animals; Antigens, Viral; Biological Sciences: Microbiology; Bromodeoxyuridine - pharmacology; Cell Line; Cell lines; Cell nucleus; Cell Transformation, Neoplastic; Cellular immunity; Culture Media; DNA, Viral - biosynthesis; Drug Resistance, Microbial; Fluorescent Antibody Technique; Fluorescent antibody techniques; Genomes; Genotype; Herpesviridae - metabolism; Human herpesvirus 4; Humans; Immune Sera; In Vitro Techniques; Lymphocytes - drug effects; Lymphocytes - enzymology; Lymphocytes - metabolism; Microscopy, Electron; Rabbits; T lymphocytes; Thymidine Kinase - metabolism; Thymine Nucleotides - metabolism; Viral antigens; Virions; Virus Replication - drug effects; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.69.1.78

The Raji line of human lymphoblastoid cells, which does not show expression of Epstein-Barr virus, was made resistant to 5-bromodeoxyuridine. Within several weeks after removal of the drug, Epstein-Barr virus particles were detected in the cells. The sequence of virus appearance in the 5-bromodeoxyuridine-resistant cells indicated drug-induced activation of a repressed Epstein-Barr virus genome.