Improved Strategies for CRISPR-Cas12-based Nucleic Acids Detection

The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology. Thus, it is urgent to develop a more simple and efficient nucleic acid detection technology. CRISPR-Cas12 has signal amplification ability, high sensitivity and high nucleic acid recognition specific...

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Bibliographic Details
Published inJournal of analysis and testing Vol. 6; no. 1; pp. 44 - 52
Main Authors Qiu, Miao, Zhou, Xiao-Ming, Liu, Lei
Format Journal Article
LanguageEnglish
Published Singapore Springer Singapore 01.03.2022
Subjects
Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Monitoring/Environmental Analysis
Review
CRISPR-Cas12
Sensitivity
Integrated detection
Simplified detection mode
Quantitative detection
Nucleic acid detection
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Summary:The COVID-19 pandemic has brought great challenges to traditional nucleic acid detection technology. Thus, it is urgent to develop a more simple and efficient nucleic acid detection technology. CRISPR-Cas12 has signal amplification ability, high sensitivity and high nucleic acid recognition specificity, so it is considered as a nucleic acid detection tool with broad development prospects and high application value. This review paper discusses recent advances in CRISPR-Cas12-based nucleic acid detection, with an emphasis on the new research methods and means to improve the nucleic acid detection capability of CRISPR-Cas12. Strategies for improving sensitivity, optimization of integrated detection, development of simplified detection mode and improvement of quantitative detection capabilities are included. Finally, the future development of CRISPR-Cas12-based nucleic acids detection is prospected.
ISSN:2096-241X
2509-4696
DOI:10.1007/s41664-022-00212-4