Towards a saturated sorghum map using RFLP and AFLP markers

• Published in: Theoretical and applied genetics Vol. 98; no. 2; pp. 320 - 328
• Main Authors: Boivin, K, Deu, M, Rami, J.F, Trouche, G, Hamon, P
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.02.1999; Berlin Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; amplified fragment length polymorphism; Barley; Biological and medical sciences; Botany; chromosome mapping; Classical and quantitative genetics; Classical and quantitative genetics. Population genetics. Molecular genetics; Datasets; Enzymes; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic aspects; genetic markers; Genetics and breeding of economic plants; Genomes; Genotype & phenotype; inbred lines; linkage (genetics); linkage groups; loci; markers; morphological markers; Morphology; Physiological aspects; Plant genetics; Polymorphism; Population genetics; recombinant inbred lines; restriction fragment length polymorphism; Sorghum; Sorghum bicolor; France; Burkina Faso; Genetic mapping; Monocotyledones; Linkage; Genetic marker; Synteny; Chromosome; Cereal crop; Sorghum bicolor; Restriction fragment length polymorphism; Gramineae; Morphology; Angiospermae; Spermatophyta
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051076

A near-saturated sorghum genetic linkage map was produced using RFLP, AFLP and morphological markers. First a composite, essentially RFLP-based genetic linkage map was obtained from analyses of two recombinant inbred populations. This map includes 343 loci for 11 linkage groups spanning 1352 cM. Since this map was constructed with many previously mapped heterologous probes, it offers a good basis for synteny studies. Separately, an AFLP map was obtained from the analysis of 168 bands revealed from 12 primer pair combinations. It includes 137 loci for 11 linkage groups spanning 849 cM. Taking into account the different data sets, we constructed a combined genetic linkage map including 443 loci spanning 1899 cM. Two main features are to be noted: (1) the distribution of AFLPs along the genome is not uniform; (2) an important stretching of the former core map is induced after adding the AFLPs.