Near infrared sensing based on fluorescence resonance energy transfer between Mn:CdTe quantum dots and Au nanorods

• Published in: Biosensors & bioelectronics Vol. 24; no. 12; pp. 3693 - 3697
• Main Authors: Liang, Guo-Xi, Pan, Hong-Cheng, Li, Ye, Jiang, Li-Ping, Zhang, Jian-Rong, Zhu, Jun-Jie
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.08.2009; Elsevier
• Subjects: Au nanorods; Biological and medical sciences; Biosensing Techniques - instrumentation; Biotechnology; Cadmium Compounds - chemistry; Equipment Design; Equipment Failure Analysis; Fluorescence resonance energy transfer; Fluorescence Resonance Energy Transfer - instrumentation; Fundamental and applied biological sciences. Psychology; Gold - chemistry; Humans; Immunoglobulin G - analysis; Manganese - chemistry; Nanotechnology - instrumentation; Nanotubes - chemistry; Nanotubes - ultrastructure; Near infrared; Quantum Dots; Spectroscopy, Near-Infrared - instrumentation; Tellurium - chemistry; Au nanorods; Fluorescence resonance energy transfer; Quantum dots; Near infrared; Near infrared radiation; Quantum dot
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2009.05.008

A novel sensing system based on the near infrared (NIR) fluorescence resonance energy transfer (FRET) between Mn:CdTe quantum dots (Qdots) and Au nanorods (AuNRs) was established for the detection of human IgG. The NIR-emitting Qdots linked with goat anti-human IgG (Mn:CdTe-Ab1) and AuNRs linked with rabbit anti-human IgG (AuNRs-Ab2) acted as fluorescence donors and acceptors, respectively. FRET occurred by human IgG with the specific antigen–antibody interaction. And human IgG was detected based on the modulation in FRET efficiency. The calibration graph was linear over the range of 0.05–2.5 μM of human IgG under optimal conditions. The proposed sensing system can decrease the interference of biomolecules in NIR region and increase FRET efficiency in optimizing the spectral overlap of AuNRs with Mn:CdTe Qdots. This method has great potential for multiplex assay with different donor–acceptor pairs.