Vitamin E Reverses Impaired Linker for Activation of T Cells Activation in T Cells from Aged C57BL/6 Mice

• Published in: The Journal of nutrition Vol. 139; no. 6; pp. 1192 - 1197
• Main Authors: Marko, Melissa G., Pang, Hoan-Jen E., Ren, Zhihong, Azzi, Angelo, Huber, Brigitte T., Bunnell, Stephen C., Meydani, Simin Nikbin
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Nutrition 01.06.2009
• Subjects: Adaptor Proteins, Signal Transducing; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Aging; Aging - physiology; animal models; Animals; Biological and medical sciences; CD4-Positive T-Lymphocytes; CD4-Positive T-Lymphocytes - drug effects; CD4-Positive T-Lymphocytes - immunology; cell proliferation; Cells, Cultured; cytology; drug effects; elderly; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; genetics; immunology; in vitro studies; Lymphocyte Activation; Lymphocyte Activation - drug effects; Lymphocyte Specific Protein Tyrosine Kinase p56(lck); Lymphocyte Specific Protein Tyrosine Kinase p56(lck) - metabolism; Membrane Proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; metabolism; Mice; Mice, Inbred C57BL; Nutritional Immunology; pharmacology; Phosphoproteins; Phosphoproteins - genetics; Phosphoproteins - metabolism; Phosphorylation; Phosphorylation - drug effects; physiology; protein kinases; Specific Pathogen-Free Organisms; Spleen; Spleen - cytology; synapse; T-lymphocytes; Vertebrates: anatomy and physiology, studies on body, several organs or systems; vitamin E; Vitamin E - pharmacology; vitamin supplements; ZAP-70 Protein-Tyrosine Kinase; ZAP-70 Protein-Tyrosine Kinase - metabolism; Micronutrient; Vertebrata; Mammalia; Nutrition; Mouse; Vitamin E; Animal; Rodentia; T-Lymphocyte; Activation; Antioxidant
• ISSN: 0022-3166; 1541-6100; 1541-6100
• DOI: 10.3945/jn.108.103416

Supplemental vitamin E alleviates age-related defects in interleukin (IL)-2 production, T cell proliferation, and immune synapse formation. Here, we evaluated the effect of in vitro supplementation with 46 mumol/L of vitamin E on T cell receptor-proximal signaling events of CD4(+) T cells from young (4-6 mo) and old (22-26 mo) C57BL mice. Aged murine CD4(+) T cells stimulated via CD3 and CD28, tyrosine 191 of the adaptor protein Linker for Activation of T cells (LAT), was hypo-phosphorylated. Supplementation with vitamin E eliminated this difference in the tyrosine phosphorylation of LAT. By using a flow cytometric assay, the age-related differences in the activation-induced phosphorylation of LAT were observed in both naïve and memory T cell subsets. In addition, supplementation with vitamin E eliminates the age-related differences in LAT phosphorylation in both T cell subsets. Neither age nor vitamin E supplementation altered the fraction of LAT entering the membrane compartment. Furthermore, neither age nor vitamin E influenced the phosphorylation of Lck and Zap70, indicating that associated changes in LAT phosphorylation were not caused by alterations in activation states of the upstream kinases Lck and Zap70.