Glucosylation of Isoeugenol and Monoterpenes in Corynebacterium glutamicum by YdhE from Bacillus lichenformis

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 9; p. 3789
• Main Authors: Ma, Su Yeong, Amoah, Obed Jackson, Nguyen, Hue Thi, Sohng, Jae Kyung
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.04.2023; MDPI
• Subjects: acetylated glucoside; Amino acids; Bacillus; Biosynthesis; Biotechnology industry; Carbon; Carbon sources; Cell culture; Corynebacterium glutamicum; Corynebacterium glutamicum - genetics; Cosmetics; Glucose; Glucosides; Glycosyltransferase; Isoeugenol; Metabolic Engineering - methods; Metabolism; Metabolites; monoterpene; Monoterpenes; NMR; Nuclear magnetic resonance; O-Glucosylation; Oils & fats; O-Glucosylation; monoterpene; acetylated glucoside; Corynebacterium glutamicum
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28093789

has been regarded as a food-grade microorganism. In recent years, the research to improve the activities of beneficial therapeutics and pharmaceutical substances has resulted in the engineering of the therapeutically favorable cell factory system of In this study, we successfully glucosylated isoeugenol and other monoterpene derivatives in using a promiscuous YdhE, which is a glycosyltransferase from . For efficient glucosylation, cultivation conditions such as the production time, substrate concentration, carbon source, and culture medium were optimized. Our system successfully converted about 93% of the isoeugenol to glucosylated compounds in the culture. The glucoside compounds were then purified, analyzed, and identified as isoeugenol-1- -β-d-glucoside and isoeugenol-1- -β-d-(2″-acetyl)-glucoside.