Near-tetraploid poorly differentiated acute myeloid leukemia M0 diagnosed by short-term cultures with a Phorbol ester TPA

• Published in: Leukemia research Vol. 18; no. 7; pp. 493 - 497
• Main Authors: Lemeẑ, Petr, Jelínek, Jaroslav, Michalová, Kyra, Koubek, Kristián, Schwarz, Jiŕí, Malasková, Vêra, Rypáčková, Blanka, Jirásek, Adam, Březinová, Jana, Hrabánek, Jan, Vítek, Antonín
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.07.1994; Elsevier Science
• Subjects: Aged; Biological and medical sciences; Cell Differentiation - drug effects; DNA, Neoplasm - analysis; Female; Hematologic and hematopoietic diseases; Humans; Immunophenotyping; Leukemia, Myeloid, Acute - diagnosis; Leukemia, Myeloid, Acute - pathology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Male; Medical sciences; MIC classification; Middle Aged; near-tetraploid leukemia; phorbol ester TPA; Polyploidy; short-term cultures; Tetradecanoylphorbol Acetate - pharmacology; Undifferentiated acute myeloid leukemia M0; phorbol ester TPA; BE, α-naphthyl butyrate esterase; PAS, the periodic acid-Schiff reagent technique; AML M0, acute myeloid poorly differentiated leukemia; MIC classification; AML, acute myeloid leukemia; ALL, acute lymphoid leukemia; short-term cultures; TPA, 12- O-tetradecanoylphorbol-13-acetatel, PE, peroxidase; AcPh, acid phosphatase; near-tetraploid leukemia; Undifferentiated acute myeloid leukemia M0; AL, acute leukemia; NAE, α-naphthyl acetate esterase; Human; Short term; Cell culture; Acute; Exploration; Malignant hemopathy; Case study; Tetraploidy; Morphological classification; Cytogenetics; Undifferentiated leukemia; Adult; Diagnosis; Elderly; Karyotype; Acute myelocytic leukemia
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/0145-2126(94)90087-6

Leukemic blasts of two patients with acute leukemia exhibited similar characteristics. They were heterogeneous in size with a diameter of 14–30 μm in smears and unclassifiable by morphological, cytochemical, immunophenotypic and ultrastructural examinations. Cytogenetic examinations of both revealed a near-tetraploid karyotype. Blasts from both patients differentiated into macrophages in cultures with 10 ng/ml 12- O-tetradecanoylphorbol-13-acetate (TPA) which is a feature specific for myeloid blasts and the cases were thus classified as poorly differentiated acute myeloid leukemias (AML M0). Near-tetraploid poorly differentiated acute myeloid leukemias M0 seem to be a special category of AML in the morphologic, immunologic and cytogenetic (MIC) classification. The presence of very large blasts in the heterogeneous blast population in acute unclassified leukemias could be a morphological sign of near-tetraploid leukemias AML M0.