Imaging individual green fluorescent proteins

Recent advances in fluorescence microscopy techniques have allowed the video-time imaging of single molecules of fluorescent dyes covalently bound to proteins in aqueous environments. However, the techniques have not been exploited fully because proteins can be difficult to label, and dye modificati...

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Bibliographic Details
Published inNature (London) Vol. 388; no. 6640; p. 338
Main Authors Pierce, Daniel W, Hom-Booher, Nora, Vale, Ronald D
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 24.07.1997
Subjects
Adenosine Triphosphate - metabolism
Dyes
Fluorescence microscopy
Green Fluorescent Proteins
Kinesin - analysis
Kinesin - genetics
Luminescent Proteins - analysis
Luminescent Proteins - genetics
Microscopy
Microscopy, Fluorescence
Mutation
Proteins
Recombinant Fusion Proteins - analysis
Recombinant Fusion Proteins - genetics
United States > US
California
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Summary:Recent advances in fluorescence microscopy techniques have allowed the video-time imaging of single molecules of fluorescent dyes covalently bound to proteins in aqueous environments. However, the techniques have not been exploited fully because proteins can be difficult to label, and dye modification may cause partial or complete loss of activity. These difficulties could be circumvented by fusing proteins to green fluorescent protein (GFP) of the jellyfish Aequorea victoria. Here we report that single S65T mutant GFP molecules can be imaged using total internal reflection microscopy, and that ATP-driven movement of an individual kinesin molecule (a microtubule motor protein) fused to GFP can be readily observed.
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ISSN:0028-0836
1476-4687
DOI:10.1038/41009