Banana disease-suppressive soil drives Bacillus assembled to defense Fusarium wilt of banana

• Published in: Frontiers in microbiology Vol. 14; p. 1211301
• Main Authors: Fan, Huacai, He, Ping, Xu, Shengtao, Li, Shu, Wang, Yongfen, Zhang, Wenlong, Li, Xundong, Shang, Hui, Zeng, Li, Zheng, Si-Jun
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 03.08.2023
• Subjects: Bacillus spp; biological control; Foc TR4; Fusarium wilt of banana; Microbiology; soil microorganism diversity
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2023.1211301

Fusarium wilt of banana (FWB) caused by Fusarium oxysporum f. sp. cubense tropical race 4 ( Foc TR4), poses a serious problem for sustainable banana production. Biological control is one of the effective measures to control this destructive disease. High-throughput sequencing of soil microorganisms could significantly improve the efficiency and accuracy of biocontrol strain screening. In this study, the soil microbial diversity of six main banana-producing areas in Yunnan was sequenced by Illumina Miseq platform. The outcome of this study showed the genus of Chujaibacter , Bacillus, and Sphingomonas were significantly enriched in microorganism community composition. Further correlation analysis with soil pathogen ( Foc TR4) content showed that Bacillus was significantly negatively correlated with pathogen content. Therefore, we isolated and identified Bacillus from the disease-suppressive soils, and obtained a B. velezensis strain YN1910. In vitro and pot experiments showed that YN1910 had a significant control effect (78.43–81.76%) on banana Fusarium wilt and had a significant growth promotion effect on banana plants.