Flow-Through Electrochemical Biosensor for the Detection of Listeria monocytogenes Using Oligonucleotides

Consumption of food contaminated by Listeria monocytogenes can result in Listeriosis, an illness with hospitalization rates of 94% and mortality rates up to 30%. As a result, U.S. regulatory agencies governing food safety retain zero-tolerance policies for L. monocytogenes. However, detection at suc...

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Published inSensors (Basel, Switzerland) Vol. 21; no. 11; p. 3754
Main Authors Armstrong, Cheryl M., Lee, Joe, Gehring, Andrew G., Capobianco, Joseph A.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 28.05.2021
MDPI
Subjects
Antibodies
Assaying
biosensor
Biosensors
Conjugation
Deoxyribonucleic acid
DNA
Electrochemical analysis
Electrodes
Enrichment
Enzymes
flow-through transducer
Food safety
foodborne pathogen
Graphite
graphite felt
Listeria
Listeria monocytogenes
Low concentrations
Pathogens
rapid detection
Sensors
Sodium
United States > US
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Summary:Consumption of food contaminated by Listeria monocytogenes can result in Listeriosis, an illness with hospitalization rates of 94% and mortality rates up to 30%. As a result, U.S. regulatory agencies governing food safety retain zero-tolerance policies for L. monocytogenes. However, detection at such low concentrations often requires strategies such as increasing sample size or culture enrichment. A novel flow-through immunoelectrochemical biosensor has been developed for Escherichia coli O157:H7 detection in 1 L volumes without enrichment. The current work further augments this biosensor’s capabilities to (1) include detection of L. monocytogenes and (2) accommodate genetic detection to help overcome limitations based upon antibody availability and address specificity errors in phenotypic assays. Herein, the conjugation scheme for oligo attachment and the conditions necessary for genetic detection are laid forth while results of the present study demonstrate the sensor’s ability to distinguish L. monocytogenes DNA from L. innocua with a limit of detection of ~2 × 104 cells/mL, which agrees with prior studies. Total time for this assay can be constrained to <2.5 h because a timely culture enrichment period is not necessary. Furthermore, the electrochemical detection assay can be performed with hand-held electronics, allowing this platform to be adopted for near-line monitoring systems.
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ISSN:1424-8220
1424-8220
DOI:10.3390/s21113754