NMR Structure of the Integral Membrane Protein OmpX
The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the Val,...
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Published in | Journal of molecular biology Vol. 336; no. 5; pp. 1211 - 1221 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
05.03.2004
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Subjects | |
Online Access | Get full text |
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Summary: | The structure of the integral membrane protein OmpX from
Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the Val, Leu, and Ile(δ
1) methyl groups in OmpX, which were selectively protonated on a perdeuterated background. The solution structure of OmpX in the DHPC micelles consists of a well-defined, eight-stranded antiparallel β-barrel, with successive pairs of β-strands connected by mobile loops. Several long-range NOEs observed outside of the transmembrane barrel characterize an extension of a four-stranded β-sheet beyond the height of the barrel. This protruding β-sheet is believed to be involved in intermolecular interactions responsible for the biological functions of OmpX. The present approach for
de novo structure determination should be quite widely applicable to membrane proteins reconstituted in mixed micelles with overall molecular masses up to about 100 kDa, and may also provide a platform for additional functional studies. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-2836 1089-8638 |
DOI: | 10.1016/j.jmb.2003.09.014 |