Cyclooxygenase-2 activates EGFR–ERK1/2 pathway via PGE2-mediated ADAM-17 signaling in testosterone-induced benign prostatic hyperplasia

• Published in: Inflammopharmacology Vol. 31; no. 1; pp. 499 - 516
• Main Authors: Abo-El Fetoh, Mohammed E., Abdel-Fattah, Maha M., Mohamed, Wafaa R., Ramadan, Laila A. A., Afify, Hassan
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.02.2023
• Subjects: ADAM17 Protein - metabolism; Allergology; Animals; Biomedical and Life Sciences; Biomedicine; Celecoxib - adverse effects; Cyclooxygenase 2 - metabolism; Dermatology; Dinoprostone - metabolism; ErbB Receptors - metabolism; Gastroenterology; Hyperplasia; Immunology; Inflammation - pathology; Male; MAP Kinase Signaling System; Original; Original Article; Pharmacology/Toxicology; Prostatic Hyperplasia - metabolism; Rats; Rats, Sprague-Dawley; Rats, Wistar; Rheumatology; Testosterone - adverse effects; Transforming Growth Factor alpha - adverse effects; Transforming Growth Factor alpha - metabolism; PGE2; Benign prostatic hyperplasia; Celecoxib; COX-2; ADAM-17 (TACE); EGFR–ERK1/2
• ISSN: 0925-4692; 1568-5608; 1568-5608
• DOI: 10.1007/s10787-022-01123-7

Objective and design Prostatic inflammation is the driving force in benign prostatic hyperplasia (BPH). This work investigated the potential modulatory effect of COX-2 inhibition on ADAM-17/EGFR/ERK1/2 axis. Materials or subjects Adult male Wistar rats were used. Treatment Celecoxib (10 and 20 mg/kg; i.p.) was injected i.p. daily for three weeks. Testosterone (TST) (3 mg/kg; s.c.) was used to induce BPH. Methods Prostatic inflammation and hyperplasia were assessed by organ weight and histopathology. Inflammatory mediators were measured using ELISA technique. Protein analysis was performed using western blotting and immunohistochemistry. Gene expression analysis was performed using qRT-PCR. Statistical analyses included one-way ANOVA and Tukey’s multiple comparison test. Results Testosterone-treated rats had a marked increase in COX-2, prostate weight, and index. Moreover, TST-induced COX-2 was inferred from cytoskeletal changes and was attributable to the overexpression of PGE2, NF-κB (p65), and IL-6. COX-2-derived PGE2 increased the activity of ADAM-17, TGF-α, and TNF-α. Consequently, EGFR–ERK1/2 pathway was over-activated, disrupting anti-apoptotic Bcl-2, cyclin D1, and pro-apoptotic Bax. Celecoxib reversed these effects. Conclusion COX-2 stimulates the ERK1/2 pathway via PGE2–ADAM-17-catalyzed shedding of TGF-α in testosterone-induced BPH. The results indicate a functional correlation between inflammation and hyperplasia in BPH.