Production of ( S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase

• Published in: Journal of bioscience and bioengineering Vol. 105; no. 4; pp. 429 - 431
• Main Authors: Kurata, Atsushi, Fujita, Michiyo, Mowafy, Amr M., Kamachi, Harumi, Kurihara, Tatsuo, Esaki, Nobuyoshi
• Format: Journal Article
• Language: English
• Published: Amsterdarm Elsevier B.V 01.04.2008; Osaka, Japan: Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands: Distributed outside Japan by Elsevier Science; Elsevier Science; Elsevier Limited
• Subjects: 2-chloroacrylate; 2-haloacrylate reductase; Acrylates - metabolism; asymmetric reduction; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Biotechnology; Burkholderia - enzymology; Burkholderia - genetics; chiral synthon; Escherichia coli - enzymology; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Glucose 1-Dehydrogenase - genetics; Glucose 1-Dehydrogenase - metabolism; Hydrocarbons, Chlorinated; Oxidation-Reduction; phenoxypropionic acid herbicides; Propionates - metabolism; S)-2-chloropropionate; 2-haloacrylate reductase; phenoxypropionic acid herbicides; S)-2-chloropropionate; asymmetric reduction; chiral synthon; 2-chloroacrylate; Enzyme; Pesticides; (S)-2-chloropropionate; Glucose 1-dehydrogenase; Herbicide; Reduction; Chirality; Production; Oxidoreductases; Reductase
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.105.429

( S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce ( S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/ l ( S)-2-chloropropionate in more than 99.9% e.e.