Production of ( S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase
( S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce ( S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an...
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Published in | Journal of bioscience and bioengineering Vol. 105; no. 4; pp. 429 - 431 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdarm
Elsevier B.V
01.04.2008
Osaka, Japan: Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands: Distributed outside Japan by Elsevier Science Elsevier Science Elsevier Limited |
Subjects | |
Online Access | Get full text |
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Summary: | (
S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce (
S)-2-chloropropionate with recombinant
Escherichia coli cells producing 2-haloacrylate reductase from
Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/
l (
S)-2-chloropropionate in more than 99.9%
e.e. |
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Bibliography: | http://dx.doi.org/10.1263/jbb.105.429 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1389-1723 1347-4421 |
DOI: | 10.1263/jbb.105.429 |