Effect of disodium cromoglycate on mast cell-mediated immediate-type allergic reactions

• Published in: Life sciences (1973) Vol. 74; no. 23; pp. 2877 - 2887
• Main Authors: Shin, Hye-Young, Kim, Jung-Sook, An, Nyeon-Hyoung, Park, Rae-Kil, Kim, Hyung-Min
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 23.04.2004
• Subjects: Administration, Oral; Anaphylaxis - chemically induced; Anaphylaxis - mortality; Anaphylaxis - prevention & control; Animals; Anti-Asthmatic Agents - administration & dosage; Anti-Asthmatic Agents - pharmacology; Cell Degranulation - drug effects; Cromolyn Sodium - administration & dosage; Cromolyn Sodium - pharmacology; Disodium cromoglycate; Dose-Response Relationship, Drug; Histamine; Histamine Release - drug effects; Longevity - drug effects; Male; Mast cells; Mast Cells - drug effects; Mast Cells - immunology; Mast Cells - pathology; Mice; Mice, Inbred ICR; p-Methoxy-N-methylphenethylamine - pharmacology; Passive cutaneous anaphylaxis; Passive Cutaneous Anaphylaxis - drug effects; Passive Cutaneous Anaphylaxis - immunology; Peritoneal Cavity - cytology; Rats; Rats, Sprague-Dawley; Survival Rate; Systemic allergic reaction; Tumor Necrosis Factor-alpha - secretion; Tumor necrosis factor-α; Histamine; Mast cells; Passive cutaneous anaphylaxis; Disodium cromoglycate; Tumor necrosis factor-α; Systemic allergic reaction
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2003.10.026

We investigated the effect of disodium cromoglycate (DSCG) on mast cell-mediated immediate-type hypersensitivity. DSCG inhibited systemic allergic reaction induced by compound 48/80 dose-dependently. Passive cutaneous anaphylaxis was inhibited by 71.6% by oral administration of DSCG (1 g/kg). When DSCG was pretreated at concentration rang from 0.01–1000 g/kg, the serum histamine levels were reduced in a dose dependent manner. DSCG also significantly inhibited histamine release from rat peritoneal mast cell (RPMC) by compound 48/80. We confirmed that DSCG inhibited compound 48/80-induced degranulation of RPMC by alcian blue/nuclear fast red staining. In addition, DSCG showed a significant inhibitory effect on anti-dinitrophenyl IgE-mediated tumor necrosis factor-α production. These results indicate that DSCG inhibits mast cell-mediated immediate-type allergic reaction.