Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs
Grape pomace (GP), a by-product in wine production, is nutritious and can be used as a feed ingredient for ruminants; however, its role in shaping sheep gastrointestinal tract (GIT) microbiota is unclear. We conducted a controlled trial using a randomized block design with 10 Tan lambs fed a control...
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Published in | Frontiers in microbiology Vol. 14; p. 1264840 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
28.09.2023
|
Subjects | |
Online Access | Get full text |
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Summary: | Grape pomace (GP), a by-product in wine production, is nutritious and can be used as a feed ingredient for ruminants; however, its role in shaping sheep gastrointestinal tract (GIT) microbiota is unclear. We conducted a controlled trial using a randomized block design with 10 Tan lambs fed a control diet (CD) and 10 Tan lambs fed a pelleted diet containing 8% GP (dry matter basis) for 46 days. Rumen, jejunum, cecum, and colon bacterial and archaeal composition were identified by 16S rRNA gene sequencing. Dry matter intake (DMI) was greater (
p
< 0.05) in the GP than CD group; however, there was no difference in average daily gain (ADG,
p
< 0.05) and feed conversion ratio (FCR,
p
< 0.05) between the two groups. The GP group had a greater abundance of
Prevotella 1
and
Prevotella 7
in the rumen; of
Sharpe
,
Ruminococcaceae 2
, and
[Ruminococcus] gauvreauii group
in the jejunum; of
Ruminococcaceae UCG-014
and
Romboutsia
in the cecum, and
Prevotella UCG-001
in the colon; but lesser
Rikenellaceae RC9 gut group
in the rumen and cecum, and
Ruminococcaceae UCG-005
and
Ruminococcaceae UCG-010
in the colon than the CD group. The pathways of carbohydrate metabolism, such as L-rhamnose degradation in the rumen, starch and glycogen degradation in the jejunum, galactose degradation in the cecum, and mixed acid fermentation and mannan degradation in the colon were up-graded; whereas, the pathways of tricarboxylic acid (TCA) cycle VIII, and pyruvate fermentation to acetone in the rumen and colon were down-graded with GP. The archaeal incomplete reductive TCA cycle was enriched in the rumen, jejunum, and colon; whereas, the methanogenesis from H
2
and CO
2
, the cofactors of methanogenesis, including coenzyme M, coenzyme B, and factor 420 biosynthesis were decreased in the colon. The study concluded that a diet including GP at 8% DM did not affect ADG or FCR in Tan lambs. However, there were some potential benefits, such as enhancing propionate production by microbiota and pathways in the GIT, promoting B-vitamin production in the rumen, facilitating starch degradation and amino acid biosynthesis in the jejunum, and reducing methanogenesis in the colon. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Anusorn Cherdthong, Khon Kaen University, Thailand Reviewed by: Chalong Wachirapakorn, Khon Kaen University, Thailand; Chaichana Suriyapha, Khon Kaen University, Thailand |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2023.1264840 |