Reliability of parylene-based multi-electrode arrays chronically implanted in adult rat brains, and evidence of electrical stimulation on contact impedance

• Published in: Journal of neural engineering Vol. 16; no. 6; p. 066047
• Main Authors: Torres-Martinez, Napoleon, Ratel, David, Crétallaz, Celine, Gaude, Christophe, Maubert, Sandrine, Divoux, Jean-Louis, Henry, Christine, Guiraud, David, Sauter-Starace, Fabien
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 19.11.2019
• Subjects: Automatic; biocompatibility; brain tissue response; chronic implantation; electrical stimulation; Engineering Sciences; Human health and pathology; impedance spectroscopy; Life Sciences; microelectrode; Neurobiology; Neurons and Cognition; thin film; Tissues and Organs; Biocompatibiliy; Chronic implantation; Microelectrode; Electrical stimulation; Impedance spectroscopy; Brain tissue response; Thin film
• ISSN: 1741-2560; 1741-2552; 1741-2552
• DOI: 10.1088/1741-2552/ab3836

Objective. The goal of this study was to evaluate the long-term behavior of the surface electrode through electrochemical characterization and follow-up of implanted parylene/platinum microelectrodes. Approach. To this aim, we designed and manufactured specific planar electrodes for cortical implantation for a rat model. This work was included in the INTENSE® project, one of the goals of which was to prove the feasibility of selective neural recording or stimulation with cuff electrodes around the vagus nerve. Main results. After a 12-week implantation in a rat model, we can report that these microelectrodes have withstood in vivo use. Regarding the biocompatibility of the electrodes (materials and manufacturing process), no adverse effect was reported. Indeed, after the three-month implantation, we characterized limited tissue reaction beneath the electrodes and showed an increase and a stabilization of their impedance. Interestingly, the follow-up of the electrochemical impedance combined with electrical stimulation highlighted a drop in the impedance up to 60% at 1 kHz after ten minutes of electrical stimulation at 110 Hz. Significance. This study gives evidence of the biocompatibility of the parylene platinum contact array designed for the project and confirms the effect of stimulation on the contact impedance.