Applications of a grid-based CYP3A4 Template system to understand the interacting mechanisms of large-size ligands; part 4 of CYP3A4 Template study

• Published in: Drug metabolism and pharmacokinetics Vol. 35; no. 6; pp. 485 - 496
• Main Authors: Goto, Takahiro, Yamazoe, Yasushi, Tohkin, Masahiro
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2020
• Subjects: Grid-base template; Large size ligands; Macrolides; Prediction of CYP3A4 metabolism; Regio-selectivity; Prediction of CYP3A4 metabolism; Macrolides; Grid-base template; Large size ligands; Regio-selectivity
• ISSN: 1347-4367; 1880-0920
• DOI: 10.1016/j.dmpk.2020.06.008

Catalytic interactions of CYP3A4 with large-size ligands have been studied on the Template established in our previous studies using polyaromatic hydrocarbon and steroid ligands (DMPK 34: 113–125 and 351–364 2019 and in press 2020). Typical CYP3A4-substrates including erythromycin, cyclosporin A (ca.1200 Da), ivermectin B1a and taxanes were applied successfully and regioselective metabolisms of these ligands were reconstituted faithfully on Template. These results suggest the applicability of CYP3A4 Template throughout broadened sizes of CYP3A4 ligands. Macrolide antibiotics showed distinct degrees of tight sittings in Width-gauge, a tool for accommodation measure. The observed differences were associated with different inhibitory/inactivation potentials of troleandomycin, erythromycin, clarithromycin and azithromycin, suggesting CYP3A4 Template also as a tool for drug-interaction mechanisms. Slight expansion of Template area was made at near Site of oxidation from simulation results of antitumor agent, rilpivirine, in the present study. Ligand entry from left side of Template is also suggested from macrolide interactions. Broadened applicability of the refined CYP3A4 Template were assured with experiments with various large-size ligands. [Display omitted]