Increased nitrotyrosine plasma levels in relation to systemic markers of inflammation and myeloperoxidase in chronic heart failure

• Published in: International journal of cardiology Vol. 135; no. 3; pp. 386 - 390
• Main Authors: Eleuteri, Ermanno, Di Stefano, Antonino, Ricciardolo, Fabio LM, Magno, Francesca, Gnemmi, Isabella, Colombo, Marilena, Anzalone, Rita, Cappello, Francesco, La Rocca, Giampiero, Genta, Francesco Tarro, Zummo, Giovanni, Giannuzzi, Pantaleo
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 10.07.2009; Elsevier
• Subjects: Aged; Biological and medical sciences; Biomarkers - blood; Cardiology. Vascular system; Cardiovascular; Chronic Disease; Cohort Studies; Endothelial dysfunction; Female; Heart; Heart Failure - blood; Heart Failure - enzymology; Heart Failure - pathology; Heart failure, cardiogenic pulmonary edema, cardiac enlargement; Humans; Inflammation Mediators - blood; Male; Medical sciences; Middle Aged; Nitrosative stress; Oxidative stress; Peroxidase - blood; Tyrosine - analogs & derivatives; Tyrosine - blood; Endothelial dysfunction; Oxidative stress; Nitrosative stress; Heart failure; Enzyme; Biological marker; Cardiovascular disease; Inflammation; Systemic; Endothelium; Blood plasma; Chronic; Peroxidases; Dysfunction; Heart disease; Level; Peroxidase; Oxidoreductases; Disseminated; Cardiology
• ISSN: 0167-5273; 1874-1754
• DOI: 10.1016/j.ijcard.2008.11.013

Abstract The presence of a reciprocal link between inflammation and oxidative/nitrosative stress has been postulated in chronic heart failure (CHF). We aimed to determine signs of nitrosative stress in serum/plasma of CHF patients. ELISA tests were used for quantification of serum/plasma levels of Nitrotyrosine (NT), H2 O2 , total NO, nitrite (NO2− ), myeloperoxidase (MPO), Tumor Necrosis Factor-alpha (TNFα) and pro-Brain Natriuretic Peptide (proBNP) in 66 CHF patients (9 in NYHA I, 34 NYHA II, 23 NYHA III) and in 14 age-matched healthy subjects. NT levels were higher in NYHA III CHF patients compared to NYHA II ( p < 0.05), NYHA I ( p < 0.03) and controls ( p < 0.02), whereas NO2− and total NO were higher in NYHA III compared to I ( p < 0.05 and p < 0.04, respectively) and controls ( p < 0.004 and 0.002) and in NYHA II compared to controls ( p < 0.04 and p < 0.009). NT levels correlated significantly with MPO ( r = 0.37, p < 0.003), TNFα ( r = 0.32, p < 0.01) and proBNP ( r = 0.32, p < 0.01). These data demonstrate an increased NT plasma level in patients with moderate/severe CHF which is associated to increased levels of markers of systemic inflammation.