Mutation profile of acute myeloid leukaemia in a Chinese cohort by targeted next‐generation sequencing

• Published in: Cancer reports Vol. 5; no. 10; pp. e1573 - n/a
• Main Authors: Lit, Benny Man Wai, Guo, Belinda B., Malherbe, Jacques A. J., Kwong, Yok Lam, Erber, Wendy N.
• Format: Journal Article
• Language: English
• Published: Hoboken John Wiley & Sons, Inc 01.10.2022; John Wiley and Sons Inc; Wiley
• Subjects: Chinese AML; Classification; DNMT3A; Genes; Genomes; Invoices; Leukemia; Mutation; mutation frequency; next‐generation sequencing; Original; Patients
• ISSN: 2573-8348; 2573-8348
• DOI: 10.1002/cnr2.1573

Background Acute myeloid leukaemia (AML) results from the clonal expansion of blast cells of myeloid origin driven by genomic defects. The advances in next‐generation sequencing (NGS) have allowed the identification of many mutated genes important in the pathogenesis of AML. Aims In this study, we aimed to assess the mutation types and frequency in a Chinese cohort presenting with de novo AML cohort using a targeted NGS strategy. Methods In total, we studied samples from 87 adult patients with de novo AML who had no prior history of cytotoxic chemotherapy. Samples were evaluated using a 120‐gene targeted NGS panel to assess the mutation profile. Results Of the 87 AML patients, there were 60 (69%) with a normal karyotype. 89.7% of patients had variants, with an average of 1.9 mutations per patient (range: 0–5 mutations per patient). DNMT3A variants were the most common, being detected in 33 patients (37.9%). NPM1 (34.5%), IDH1/2 (24.1%) and FLT3‐ITD (20.7%) mutations was the next most common. Of the patients with DNMT3A mutations, 24.2% also had mutations NPM1 and FLT3‐ITD and 6.1% NPM1, FLT3‐ITD and IDH mutations. Conclusion Both DNMT3A and NPM1 mutations were more common than in other Chinese and Western AML cohorts that have been studied. DNMT3A mutations tended to co‐occur with NPM1 and FLT3‐ITD mutations and were most commonly seen with a normal karyotype.