Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

• Published in: Nucleic acids research Vol. 39; no. 7; pp. 2671 - 2677
• Main Authors: Munkley, Jennifer, Copeland, Nikki A., Moignard, Victoria, Knight, John R. P., Greaves, Erin, Ramsbottom, Simon A., Pownall, Mary E., Southgate, Jennifer, Ainscough, Justin F.-X., Coverley, Dawn
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.2011
• Subjects: Active Transport, Cell Nucleus; Animals; Cell Differentiation; Cell Line, Tumor; Cells, Cultured; Cyclin E - metabolism; cyclins; DNA replication; embryonic stem cells; Humans; Mice; Molecular Biology; neoplasm cells; neoplasms; Neoplasms - metabolism; nuclear matrix; Nuclear Matrix - metabolism; nucleic acids; Protein Transport; Xenopus; Xenopus laevis

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In mu...