Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells

Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In mu...

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Published inNucleic acids research Vol. 39; no. 7; pp. 2671 - 2677
Main Authors Munkley, Jennifer, Copeland, Nikki A., Moignard, Victoria, Knight, John R. P., Greaves, Erin, Ramsbottom, Simon A., Pownall, Mary E., Southgate, Jennifer, Ainscough, Justin F.-X., Coverley, Dawn
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.04.2011
Subjects
Active Transport, Cell Nucleus
Animals
Cell Differentiation
Cell Line, Tumor
Cells, Cultured
Cyclin E - metabolism
cyclins
DNA replication
embryonic stem cells
Humans
Mice
Molecular Biology
neoplasm cells
neoplasms
Neoplasms - metabolism
nuclear matrix
Nuclear Matrix - metabolism
nucleic acids
Protein Transport
Xenopus
Xenopus laevis
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Summary:Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.
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ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gkq1190