A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead. The hi...

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Published inBrazilian archives of biology and technology Vol. 54; no. 6; pp. 1175 - 1186
Main Authors Acevedo, Francisca, Pizzul, Leticia, Castillo, María del Pilar, Rubilar, Olga, Lienqueo, María Elena, Tortella, Gonzalo, Diez, María Cristina
Format Journal Article
LanguageEnglish
Published Instituto de Tecnologia do Paraná (Tecpar) 01.12.2011
Subjects
Annan biologi
ligninolytic enzymes
lignocellulosic material
Other Biological Topics
purification
white-rot fungi
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Summary:In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead. The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO 4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50°C) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
ISSN:1516-8913
1678-4324
1516-8913
1678-4324
DOI:10.1590/S1516-89132011000600013