Generation and Validation of Monoclonal Antibodies Suitable for Detecting and Monitoring Parvovirus Infections

• Published in: Pathogens (Basel) Vol. 11; no. 2; p. 208
• Main Authors: Tessmer, Claudia, Plotzky, Claudia, Fees, Jana, Welsch, Hendrik, Eudenbach, Rebecca, Faber, Martin, Simón, Alicia, Angelova, Assia, Rommelaere, Jean, Hofmann, Ilse, Nüesch, Jürg P F
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.02.2022; MDPI
• Subjects: Anticancer properties; Antitumor agents; Binding sites; Cancer; Cell cycle; Chronic illnesses; Cross-reactivity; diagnostic tools; generation of monoclonal antibodies for recognizing parvoviral NS1 proteins; Genomes; Human tissues; Immunofluorescence; Immunology; Infections; Kinases; Localization; Monitoring; Monoclonal antibodies; NS1; Parvoviruses; Polypeptides; Primates; Proteins; Rodents; Species; Target detection; Viruses; generation of monoclonal antibodies for recognizing parvoviral NS1 proteins; parvoviruses; NS1; diagnostic tools; monoclonal antibodies
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens11020208

For many applications it is necessary to detect target proteins in living cells. This is particularly the case when monitoring viral infections, in which the presence (or absence) of distinct target polypeptides potentially provides vital information about the pathology caused by the agent. To obtain suitable tools with which to monitor parvoviral infections, we thus generated monoclonal antibodies (mAbs) in order to detect the major non-structural protein NS1 in the intracellular environment and tested them for sensitivity and specificity, as well as for cross-reactivity towards related species. Using different immunogens and screening approaches based on indirect immunofluorescence, we describe here a panel of mAbs suitable for monitoring active infections with various parvovirus species by targeting the major non-structural protein NS1. In addition to mAbs detecting the NS1 of parvovirus H-1 (H-1PV) (belonging to the species, which is currently under validation as an anti-cancer agent), we generated tools with which to monitor infections by human cutavirus (CuV) and B19 virus (B19V) (belonging to the and the species, respectively, which were both found to persistently infect human tissues). As well as mAbs able to detect NS1 from a broad range of parvoviruses, we obtained entities specific for either (distinct) members of the species, human CuV, or human B19V.