Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: Effects of oral doses of AKG. A proteomic approach

• Published in: General and comparative endocrinology Vol. 169; no. 3; pp. 284 - 292
• Main Authors: Ibarz, Antoni, Costa, Rita, Harrison, Adrian P, Power, Deborah M
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2010
• Subjects: Animals; Diet; Endocrinology & Metabolism; Feedback; Ketoglutaric Acids - pharmacology; Marine; Neuroendocrine; Pituitary Gland - drug effects; Pituitary Gland - physiology; Pituitary proteome; Protein Folding - drug effects; Proteome - drug effects; Sea Bream - metabolism; Somatolactin; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Somatolactin; Neuroendocrine; Pituitary proteome
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2010.09.010

Research highlights ► The paper is original and examines the influence of a daily oral dose of alpha-ketoglutarate (a dietary additive) and resulting molecular modifications by a proteomic approach in pituitary gilthead sea bream. ► For the first time a fish pituitary proteome was obtained and dietary AKG multifactorial alterations were related with a feed-back (nutrition to pituitary) loop. ► The pituitary proteome revealed up-regulation of proteins related to glucose metabolism and protein folding, and down-regulation of somatolactin and apolipoprotein (the most abundant protein in pituitary proteome). ► Moreover, some interesting proteins related to neural regeneration (such as cofilin and Vat-protein) were up-regulated. ► These results provide evidence of a potential endocrine/metabolic regulatory loop activated by AKG supplementation.