Experimental design for single-cell RNA sequencing

Abstract Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most...

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Bibliographic Details
Published inBriefings in functional genomics Vol. 17; no. 4; pp. 233 - 239
Main Authors Baran-Gale, Jeanette, Chandra, Tamir, Kirschner, Kristina
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.07.2018
Subjects
chromium
experimental design
Gene Expression Profiling - methods
Microfluidic Analytical Techniques
sequence analysis
Sequence Analysis, RNA - methods
Single-Cell Analysis - methods
single-cell RNA sequencing
Smart-Seq 2
Drop-Seq
experimental design
10x Chromium
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Summary:Abstract Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.
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SourceType-Scholarly Journals-1
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ISSN:2041-2649
2041-2657
2041-2657
DOI:10.1093/bfgp/elx035