Identification of the Fcα/μR isoform specifically expressed in the kidney tubules

• Published in: Molecular immunology Vol. 46; no. 4; pp. 749 - 753
• Main Authors: Kurita, Naoki, Honda, Shin-ichiro, Usui, Kenta, Shimizu, Yoshio, Miyamoto, Akitomo, Tahara-Hanaoka, Satoko, Shibuya, Kazuko, Shibuya, Akira
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.02.2009; Elsevier
• Subjects: Fc receptor; Fcα/μR; IgA; IgA nephropathy; IgM; IgA; Fc receptor; IgA nephropathy; Fcα/μR; IgM
• ISSN: 0161-5890; 1872-9142
• DOI: 10.1016/j.molimm.2008.10.002

Fcα/μR is expressed not only in lymphoid, but also in non-lymphoid organs, including kidney. However, molecular and functional characteristics of Fcα/μR, particularly expressed in non-lymphoid organs, have remained unclear. Here we identified an isoform of Fcα/μR in the murine kidney on the C57BL/6J background. The kidney expressed only the isoform, which was not expressed in other lymphoid and non-lymphoid organs, and this isoform binds both IgA and IgM. Immunohistochemical analyses suggested that the Fcα/μR isoform was expressed in the tubular epithelial cells, but not in the glomeruli. This was confirmed by flow cytometry analysis of isolated tubular epithelial cells and by RT-PCR analyses using the separately excised glomerular and tubular regions by the laser microdissection system. These results suggest that Fcα/μR may not be involved in IgA deposition in glomerular mesangium cells in IgA nephropathy. Rather, it may play an important role in immunity in the renal tubular regions.