CRISPR-Cas13: A new technology for the rapid detection of pathogenic microorganisms

Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA to produce a sensitive and specif...

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Published inFrontiers in microbiology Vol. 13; p. 1011399
Main Authors Huang, Zhanchao, Fang, Jianhua, Zhou, Min, Gong, Zhenghua, Xiang, Tianxin
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 28.10.2022
Subjects
Cas13
CRISPR
Microbiology
microorganisms
pathogenic
rapid detection
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Summary:Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs programmable RNA to produce a sensitive and specific method with high base resolution and thus to provide a novel tool for the rapid detection of microorganisms. The review aims to provide insights to spur further development by summarizing the characteristics of effectors of the CRISPR-Cas13 system and by describing the latest research into its application in the rapid detection of pathogenic microorganisms in combination with nucleic acid extraction, isothermal amplification, and product detection.
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This article was submitted to Microbiotechnology, a section of the journal Frontiers in Microbiology
Edited by: Dongsheng Zhou, Beijing Institute of Microbiology and Epidemiology, China
Reviewed by: Mohammad Enamul Hoque Kayesh, Patuakhali Science and Technology University, Bangladesh; Sandra Coral Garrett, UCONN Health, United States
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2022.1011399