Report into a discordant result at D16S539 between SGM Plus® and PowerPlex® ESI 16 kits in a criminal case sample and implications for the UK National DNA Database upgrade

Abstract Upon re-testing of a DNA extract as part of a defence examination, a discordant result was observed at D16S539. Further STR testing and DNA sequencing of the sample identified the cause as a primer binding site mutation which was shown to be a previously unreported SNP. The testing results...

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Bibliographic Details
Published inScience & justice Vol. 54; no. 1; pp. 95 - 97
Main Authors Revoir, A, Ballard, D.J, Syndercombe Court, D
Format Journal Article
LanguageEnglish
Published England Elsevier Ireland Ltd 01.01.2014
Forensic Science Society
Subjects
Binding sites
D16S539
Databases, Genetic
Deoxyribonucleic acid
Discordance
DNA
DNA Fingerprinting - instrumentation
Forensic sciences
Humans
Microsatellite Repeats
Multiplex Polymerase Chain Reaction
Multiplex Upgrade Project
Mutation
Next generation multiplex kits
Pathology
Sequence Analysis, DNA
UK National DNA database
United Kingdom > UK
Multiplex Upgrade Project
D16S539
Discordance
UK National DNA database
Next generation multiplex kits
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Summary:Abstract Upon re-testing of a DNA extract as part of a defence examination, a discordant result was observed at D16S539. Further STR testing and DNA sequencing of the sample identified the cause as a primer binding site mutation which was shown to be a previously unreported SNP. The testing results obtained in this case are considered in light of the current ongoing Multiplex Upgrade Project in the UK and the likely increase in discordant results that may be observed once different next generation kits are introduced.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1355-0306
1876-4452
DOI:10.1016/j.scijus.2013.08.005