Enhancement of Swimming Speed Leads to a More-Efficient Chemotactic Response to Repellent

• Published in: Applied and environmental microbiology Vol. 82; no. 4; pp. 1205 - 1214
• Main Authors: Karmakar, Richa, Uday Bhaskar, R V S, Jesudasan, Rajesh E, Tirumkudulu, Mahesh S, Venkatesh, K V
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 15.02.2016
• Subjects: Chemotaxis; E coli; Environmental Microbiology; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - physiology; Locomotion; Microbiology; Models, Biological; Nickel - metabolism; Velocity
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.03397-15

Negative chemotaxis refers to the motion of microorganisms away from regions with high concentrations of chemorepellents. In this study, we set controlled gradients of NiCl2, a chemorepellent, in microchannels to quantify the motion of Escherichia coli over a broad range of concentrations. The experimental technique measured the motion of the bacteria in space and time and further related the motion to the local concentration profile of the repellent. Results show that the swimming speed of bacteria increases with an increasing concentration of repellent, which in turn enhances the drift velocity. The contribution of the increased swimming speed to the total drift velocity was in the range of 20 to 40%, with the remaining contribution coming from the modulation of the tumble frequency. A simple model that incorporates receptor dynamics, including adaptation, intracellular signaling, and swimming speed variation, was able to qualitatively capture the observed trend in drift velocity.