Type 2 heat-labile enterotoxin (LT-II)-producing Escherichia coli isolated from ostriches with diarrhea

• Published in: Veterinary microbiology Vol. 105; no. 3; pp. 245 - 249
• Main Authors: Nardi, Ana Rita M., Salvadori, Márcia R., Coswig, Lia T., Gatti, Maria Sílvia V., Leite, Domingos S., Valadares, Geórgio F., Neto, Manoel Garcia, Shocken-Iturrino, Rubén P., Blanco, Jesús E., Yano, Tomomasa
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.02.2005; Elsevier Science
• Subjects: Animals; Bacterial Toxins - biosynthesis; Bacteriology; Biological and medical sciences; bird diseases; Bird Diseases - microbiology; Chlorocebus aethiops; CHO Cells; Cricetinae; Cricetulus; cytoplasm; Cytoplasmic vacuolation; cytotoxicity; cytotoxins; Diarrhea; Diarrhea - microbiology; Diarrhea - veterinary; digestive system diseases; enteropathogenic Escherichia coli; enterotoxins; Enterotoxins - biosynthesis; Escherichia coli; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli - metabolism; Escherichia coli Proteins - biosynthesis; Escherichia infections; feces; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; heat stability; isolation; Microbiology; Miscellaneous; Ostrich; ostriches; pathogenicity; Struthio; Struthioniformes - microbiology; Type 2 heat-labile enterotoxin; Type 2 heat-labile enterotoxin (LT-II); vacuoles; Vero Cells; Brazil; Diarrhea; Type 2 heat-labile enterotoxin (LT-II); Ostrich; Escherichia coli; Cytoplasmic vacuolation; Microbiology; Toxin; Enterotoxin; Digestive diseases; Bacteria; Intestinal disease; Veterinary; Enterobacteriaceae
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2004.11.005

The culture supernatant of Escherichia coli, isolated from ostriches with diarrhea in Brazil, caused elongation in Vero cell, rounding in Chinese hamster ovary (CHO) cells and a cytoplasmic vacuolation in ostrich embryo fibroblasts (OEF), but it was not cytotoxic for chicken embryo fibroblasts (CEF). These effects were not neutralized by antiserum to cholera toxin. Polymerase chain reaction assays showed that the ostrich E. coli contained the gene encoding ( eltII-A), but not those for type 1 heat-labile enterotoxin ( eltA), heat-stable enterotoxins ( estA, estB), verocytotoxins ( stx-I, stx-II), or cytotoxic necrotizing factors ( cnf 1, cnf 2). All isolates belonged to serotype O15:H8. The enteropathogenic relevance of LT-II in ostrich diarrhea remains undetermined.