Structural analysis of dansyl glyco-asparagines from quail ovalbumin

The carbohydrate moiety of quail ovalbumin was isolated in the form of dansyl glyco-asparagines. The various components were separated by high-performance liquid chromatography, yielding two major fractions (90% of the total carbohydrate material). By 500-MHz 1H-NMR spectroscopy they were found to b...

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Published inBiochimica et biophysica acta Vol. 843; no. 1; pp. 123 - 127
Main Authors Mutsaers, Johanna H.G.M., van Halbeek, Herman, Vliegenthart, Johannes F.G., Iwase, Hitoo, Kato, Yukinobu, Hotta, Kyoko
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.11.1985
Elsevier
North-Holland
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrate analysis
Dansyl glyco-asparagine (Quail)
Fundamental and applied biological sciences. Psychology
Glycoproteins
Ovalbumin
Proteins
Ovalbumin
Dansyl glyco-asparagine (Quail)
DNS
GlcNAc
Man
Carbohydrate analysis
ODS
Vertebrata
Molecular structure
HPLC chromatography
Glycosylation
NMR spectrometry
Aves
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Summary:The carbohydrate moiety of quail ovalbumin was isolated in the form of dansyl glyco-asparagines. The various components were separated by high-performance liquid chromatography, yielding two major fractions (90% of the total carbohydrate material). By 500-MHz 1H-NMR spectroscopy they were found to be of the oligomannoside type, containing eight and seven d-mannose residues. The largest one possesses the following structure: ▪ while the other one is its analogue missing mannose D 1. Dansyl glyco-asparagines turned out to be suitable derivatives for 1H-NMR spectroscopic analysis; in combination with HPLC, heterogeneity of sugar chains on glycoproteins can be elegantly characterized.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(85)90058-3