Glucoamylase production in batch, chemostat and fed-batch cultivations by an industrial strain of Aspergillus niger

• Published in: Applied microbiology and biotechnology Vol. 53; no. 3; pp. 272 - 277
• Main Authors: PEDERSEN, H, BEYER, M, NIELSEN, J
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.03.2000; Springer Nature B.V
• Subjects: Aspergillus niger; Aspergillus niger - enzymology; Aspergillus niger - growth & development; Bacteria; Biological and medical sciences; Biomass; Biotechnology; Biotechnology - methods; Carbon - metabolism; Carbon sources; Culture Media; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; Glucan 1,4-alpha-Glucosidase - biosynthesis; Glucan 1,4-alpha-Glucosidase - genetics; Growth conditions; maltodextrin; maltodextrins; maltose; Methods. Procedures. Technologies; Microbiology; Production of selected enzymes; Glucan 1,4-α-glucosidase; Microorganism growth; Enzyme; Carbon; Fungi; Continuous process; Batchwise; Nutrient source; Aspergillus niger; Glycosidases; Production; Microorganism culture; Hydrolases; Fungi Imperfecti; Semicontinuous; Thallophyta
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s002530050020

The Aspergillus niger strain BO-1 was grown in batch, continuous (chemostat) and fed-batch cultivations in order to study the production of the extracellular enzyme glucoamylase under different growth conditions. In the pH range 2.5-6.0, the specific glucoamylase productivity and the specific growth rate of the fungus were independent of pH when grown in batch cultivations. The specific glucoamylase productivity increased linearly with the specific growth rate in the range 0-0.1 h(-1) and was constant in the range 0.1-0.2 h(-1). Maltose and maltodextrin were non-inducing carbon sources compared to glucose, and the maximum specific growth rate was 0.19 +/- 0.02 h(-1) irrespective of whether glucose or maltose was the carbon source. In fed-batch cultivations, glucoamylase titres of up to 6.5 g l(-1) were obtained even though the strain contained only one copy of the glaA gene.