Bioaugmentation of a 4-chloronitrobenzene contaminated soil with Pseudomonas putida ZWL73

• Published in: Environmental pollution (1987) Vol. 157; no. 3; pp. 763 - 771
• Main Authors: Niu, Gui-Lan, Zhang, Jun-Jie, Zhao, Shuo, Liu, Hong, Boon, Nico, Zhou, Ning-Yi
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.03.2009; Elsevier
• Subjects: 4-Chloronitrobenzene; Acidobacteria; Actinobacteria; Actinobacteria - physiology; alpha-Proteobacteria; Alphaproteobacteria - physiology; ammonia; Ammonia - analysis; Ammonia-oxidizing bacteria; Animal, plant and microbial ecology; Applied ecology; bacterial contamination; beta-Proteobacteria; Betaproteobacteria - physiology; bioaccumulation; Bioaugmentation; biodegradation; Biodegradation, Environmental; Biological and medical sciences; biological resistance; chlorides; Chlorides - analysis; Colony Count, Microbial; Denaturing gradient gel electrophoresis; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; General aspects; heterotrophs; microbial ecology; Nitrobenzenes - metabolism; Nitrosomonadaceae; Pseudomonas putida; Pseudomonas putida - metabolism; Pseudomonas putida ZWL73; Soil Microbiology; soil microorganisms; Soil Pollutants - metabolism; soil pollution; species differences; Terrestrial environment, soil, air; 4-Chloronitrobenzene; Denaturing gradient gel electrophoresis; Bioaugmentation; Pseudomonas putida ZWL73; Ammonia-oxidizing bacteria; Pseudomonadales; Soil pollution; Ammonia; Decontamination; Pseudomonadaceae; Bacteria; Environment; Ecological recovery; Bioremediation; Pseudomonas putida
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2008.11.024

The strain Pseudomonas putida ZWL73, which metabolizes 4-chloronitrobenzene (4CNB) by a partial-reductive pathway, was inoculated into lab-scale 4CNB-contaminated soil for bioaugmentation purposes in this study. The degradation of 4CNB was clearly stimulated, as indicated with the gradual accumulation of ammonium and chloride. Simultaneously, the diversity and quantity of cultivable heterotrophic bacteria decreased due to 4CNB contamination, while the quantity of 4CNB-resistant bacteria increased. During the bioaugmentation, denaturing gradient gel electrophoresis analysis showed the changes of diversity in dominant populations of intrinsic soil microbiota. The results showed that Alphaproteobacteria and Betaproteobacteria were not distinctly affected, but Actinobacteria were apparently stimulated. In addition, an interesting dynamic within Acidobacteria was observed, as well as an influence on ammonia-oxidizing bacteria population. These combined findings demonstrate that the removal of 4CNB in soils by inoculating strain ZWL73 is feasible, and that specific populations in soils rapidly changed in response to 4CNB contamination and subsequent bioaugmentation. Pseudomonas putida ZWL73 can accelerate 4CNB removal in lab-scale soils, causing dynamic changes within intrinsic Actinobacteria and Acidobacteria.