Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients-Comparison with the Standard Histopathological Procedure

The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical rele...

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Published inCells (Basel, Switzerland) Vol. 9; no. 12; p. 2611
Main Authors Vodicka, Josef, Pesta, Martin, Kulda, Vlastimil, Houfkova, Katerina, Vankova, Bohuslava, Sebek, Jakub, Skala, Martin, Fichtl, Jakub, Prochazkova, Kristyna, Topolcan, Ondrej
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 04.12.2020
MDPI
Subjects
Cytokeratin
H&amp
IHC CK19
Immunohistochemistry
Lung cancer
Lymph nodes
Lymphatic system
Medical prognosis
Metastasis
Methods
mRNA
OSNA
Patients
Statistical analysis
Survival analysis
Tumors
Japan
lung cancer
IHC CK19
OSNA
lymph nodes
E
H&amp
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Summary:The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4-5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells9122611