Discrimination of polysorbate 20 by high-performance liquid chromatography-charged aerosol detection and characterization for components by expanding compound database and library

• Published in: Journal of pharmaceutical analysis Vol. 14; no. 5; pp. 100929 - 11
• Main Authors: Wang, Shi-Qi, Zhao, Xun, Zhang, Li-Jun, Zhao, Yue-Mei, Chen, Lei, Zhang, Jin-Lin, Wang, Bao-Cheng, Tang, Sheng, Yuan, Tom, Yuan, Yaozuo, Zhang, Mei, Lee, Hian Kee, Shi, Hai-Wei
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.05.2024; Xi'an Jiaotong University, Journal of Pharmaceutical Analysis; Elsevier
• Subjects: Aerosols; Chromatography; Component; Degradation; Discrimination; Esters; Fatty acids; High-performance liquid chromatography; Investigations; Libraries; Manufacturers; Manufacturing; Mass spectrometry; Mass spectroscopy; NMR; Nuclear magnetic resonance; Polymerization; Polysorbate 20; Scientific imaging; Degradation; Database; Discrimination; Component; Polysorbate 20
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2023.12.019

Analyzing polysorbate 20 (PS20) composition and the impact of each component on stability and safety is crucial due to formulation variations and individual tolerance. The similar structures and polarities of PS20 components make accurate separation, identification, and quantification challenging. In this work, a high-resolution quantitative method was developed using single-dimensional high-performance liquid chromatography (HPLC) with charged aerosol detection (CAD) to separate 18 key components with multiple esters. The separated components were characterized by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) with an identical gradient as the HPLC-CAD analysis. The polysorbate compound database and library were expanded over 7-time compared to the commercial database. The method investigated differences in PS20 samples from various origins and grades for different dosage forms to evaluate the composition-process relationship. UHPLC-Q-TOF-MS identified 1329 to 1511 compounds in 4 batches of PS20 from different sources. The method observed the impact of 4 degradation conditions on peak components, identifying stable components and their tendencies to change. HPLC-CAD and UHPLC-Q-TOF-MS results provided insights into fingerprint differences, distinguishing quasi products. [Display omitted] •Distinguish subtle differences of PS20 samples from various origins.•Expand polysorbate compound database by over 7 times for precise characterization.•Detailed PS20 composition profile was established.•Allows study of composition changes and influencing factors during degradation.