Evaluation of Brucella abortus DNA and RNA vaccines expressing Cu–Zn superoxide dismutase (SOD) gene in cattle

• Published in: Veterinary microbiology Vol. 129; no. 3; pp. 396 - 403
• Main Authors: Sáez, Darwin, Guzmán, Ingrid, Andrews, Edilia, Cabrera, Alex, Oñate, Angel
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 22.06.2008; Amsterdam; New York: Elsevier; Elsevier Science
• Subjects: Animals; Animals, Newborn; Antibodies, Bacterial - biosynthesis; Antibody Formation; Bacteriology; Biological and medical sciences; Bovine; Brucella abortus; Brucella abortus - enzymology; Brucella abortus - genetics; Brucella abortus - immunology; Brucella melitensis biovar Abortus; Brucella Vaccine - administration & dosage; Brucella Vaccine - immunology; Brucellosis, Bovine - prevention & control; Cattle; copper; Cu–Zn superoxide dismutase; DNA; DNA vaccines; drug evaluation; Female; Fundamental and applied biological sciences. Psychology; gene expression; Genetic vaccine; immune response; Immunity, Cellular; immunogenetics; interleukin-4; Microbiology; Miscellaneous; Random Allocation; RNA - immunology; RNA vaccines; Semliki Forest virus; superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - immunology; Superoxide Dismutase - metabolism; tumor necrosis factor-alpha; vaccination; vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology; zinc; Cu–Zn superoxide dismutase; Brucella abortus; Bovine; Genetic vaccine; Cu-Zn superoxide dismutase; Microbiology; Enzyme; Superoxide dismutase; Vertebrata; Mammalia; Brucellaceae; Gene; Bacteria; Artiodactyla; Oxidoreductases; Veterinary; Ungulata
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2007.11.015

This study was conducted to evaluate the immunogenicity of a DNA or RNA vaccines encoding Brucella abortus Cu–Zn superoxide dismutase (SOD) in cattle. Intramuscular injection of plasmid DNA carrying Brucella SOD gene (pcDNA-SOD) into animals elicited both humoral and cellular immune responses. Animals injected with pcDNA-SOD developed SOD IgG antibody with predominance of immunoglobulin G1 (IgG1) isotype over IgG2. In addition, the DNA vaccine elicited a specific T-cell-proliferative response. Furthermore, intraperitoneal injection of cattle with recombinant Semliki Forest virus particles carrying recombinant RNA encoding SOD (SFV-SOD) did not lead to the induction of SOD IgG 1 or 2 antibody, but induced specific T-cell activation. Both vaccines were able to induce a non-significant secretion of gamma interferon and did not induce the secretion of IL-4 or tumor necrosis factor (TNF)-α. These results suggest that SOD gene in a genetic vaccine formulation (DNA or RNA) might be of potential us as a vaccine to induce cell-mediated immunity in cattle. To our knowledge, this is the first study to evaluate a genetic vaccine against Brucella in cattle.