Regulation of two homodimer hexosaminidases in the mycoparasitic fungus Trichoderma asperellum by glucosamine

• Published in: Current genetics Vol. 45; no. 4; pp. 205 - 213
• Main Authors: Ramot, O, Viterbo, A, Friesem, D, Oppenheim, A, Chet, I
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.04.2004
• Subjects: Bacteria; beta-N-acetylhexosaminidase; Biological control; biological control agents; Chitin; Dimerization; enzyme activity; Enzymes; EXC1Y protein; gene expression regulation; Gene Expression Regulation, Fungal - physiology; gene induction; genes; glucosamine; Glucosamine - metabolism; Hexosaminidases - genetics; Hexosaminidases - metabolism; Hexosaminidases - secretion; Mass spectrometry; Microbiology; molecular sequence data; mycoparasites; nucleotide sequences; Pathogens; promoter regions; Promoter Regions, Genetic; Regulation; Sequence Analysis, DNA; Trichoderma; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma - metabolism; Trichoderma asperellum
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/s00294-003-0478-0

Trichoderma asperellum is a mycoparasitic fungus which is used as a biocontrol agent against plant pathogens. Its hydrolytic enzymes take part in its parasitic interaction, degrading the pathogen cell wall and thereby helping to control disease. One of those enzymes, beta- N-acetyl- d-glucosaminidase (GlcNAcase), degrades chitin, which is a major component of the cell wall of many plant-pathogenic fungi. Two GlcNAcases of T. asperellum T203, designated EXC1Y and EXC2Y, were purified, their genes and their promoters were sequenced, and their regulation was studied. The enzymes share homology (59% identity) but are easily distinguished by PAGE assay. Biochemical characterization, Edman degradation, and mass spectrometry demonstrated that EXC1Y and EXC2Y are both active as homodimers. Both genes are up-regulated by glucosamine (GlcN), in contrast to two endochitinases of this fungus. GlcN induces the secretion of several proteins (including a beta-glucosidase), among which EXC1Y is the most abundant. An exc2y knockout was constructed, to study the regulation of EXC1Y expression and secretion. The fungus has the ability to store a high amount of this enzyme in an active form and secrete it into the medium later.