Mortality of cutworm larvae is not enhanced by Agrotis segetum granulovirus and Agrotis segetum nucleopolyhedrovirus B coinfection relative to single infection by either virus

• Published in: Applied and Environmental Microbiology Vol. 81; no. 8; pp. 2893 - 2899
• Main Authors: Wennmann, Jörg T, Köhler, Tim, Gueli Alletti, Gianpiero, Jehle, Johannes A
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.04.2015
• Subjects: Agrotis segetum; Animals; Baculovirus; Biological Control Agents - standards; Coinfection - virology; Granulovirus; Granulovirus - physiology; Insects; Invertebrate Microbiology; Larva - growth & development; Larva - virology; Mortality; Moths - growth & development; Moths - virology; Nuclear polyhedrosis virus; Nucleopolyhedrovirus - physiology; Polymerase chain reaction; Viruses
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.03726-14

Mixed infections of insect larvae with different baculoviruses are occasionally found. They are of interest from an evolutionary as well as from a practical point of view when baculoviruses are applied as biocontrol agents. Here, we report mixed-infection studies of neonate larvae of the common cutworm, Agrotis segetum, with two baculoviruses, Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV). By applying quantitative PCR (qPCR) analysis, coinfections of individual larvae were demonstrated, and occlusion body (OB) production within singly infected and coinfected larvae was determined in individual larvae. Mixtures of viruses did not lead to changes in mortality rates compared with rates of single-virus treatments, indicating an independent action within host larvae under our experimental conditions. AgseNPV-B-infected larvae showed an increase in OB production during 2 weeks of infection, whereas the number of AgseGV OBs did not change from the first week to the second week. Fewer OBs of both viruses were produced in coinfections than in singly infected larvae, suggesting a competition of the two viruses for larval resources. Hence, no functional or economic advantage could be inferred from larval mortality and OB production from mixed infections of A. segetum larvae with AgseNPV-B and AgseGV.