Primo-vessels and Primo-nodes in Rat Brain, Spine and Sciatic Nerve

• Published in: Journal of acupuncture and meridian studies Vol. 3; no. 2; pp. 111 - 115
• Main Authors: Lee, Byung-Cheon, Eom, Ki-Hoon, Soh, Kwang-Sup
• Format: Journal Article
• Language: English
• Published: Korea (South) 사단법인약침학회 01.06.2010
• Subjects: Acupuncture Points; Animals; arachnoid mater; Blood Vessels - chemistry; brain; Brain - blood supply; Brain Chemistry; Male; Meridians; primo-node (Bonghan corpuscle); primo-vessel (Bonghan duct); Rats; Rats, Sprague-Dawley; sciatic nerve; Sciatic Nerve - blood supply; Sciatic Nerve - chemistry; spinal cord; Spinal Cord - blood supply; Spinal Cord - chemistry; Staining and Labeling; Trypan blue; Trypan Blue - analysis; 한의학; primo-node (Bonghan corpuscle); Trypan blue; arachnoid mater; spinal cord; brain; primo-vessel (Bonghan duct); sciatic nerve
• ISSN: 2005-2901; 2093-8152
• DOI: 10.1016/S2005-2901(10)60020-9

We report a method using Trypan blue staining to detect primo-vessels in the nervous system on internal organs or in the skin of rat. We applied this technique to visualize the primo-vessels and primo-nodes in the brain, spinal cord and sciatic nerve of a rat. Primo-vessels and primo-nodes were preferentially stained at nerves, blood vessels, or fascia-like membranes and turned blue after the spread and washing of Trypan blue. The physiological role of the primo-vessels within the nervous system is an important question warranting further investigation.