The artificial humic substance HS1500 does not inhibit photosynthesis of the green alga Desmodesmus armatus in vivo but interacts with the photosynthetic apparatus of isolated spinach thylakoids in vitro

• Published in: Photosynthesis research Vol. 137; no. 3; pp. 403 - 420
• Main Authors: Gilbert, Matthias, Bährs, Hanno, Steinberg, Christian E. W., Wilhelm, Christian
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2018; Springer; Springer Nature B.V
• Subjects: Algae; Analysis; Biochemistry; Biomedical and Life Sciences; Chlorophyll; Desmodesmus armatus; Electron transport; Fluorescence; Herbicides; Humic acids; Life Sciences; Nutrient availability; Original Article; Oxygen consumption; Phenolic compounds; Photosynthesis; Photosynthetic apparatus; Photosystem I; Photosystem II; Physiological aspects; Physiology; Phytoplankton; Plankton; Plant biochemistry; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Spinacia oleracea; Thylakoids; Photosynthetic oxygen evolution; Chlorophyll fluorescence; Algicidal/herbicidal function; Algal growth; Humic substances; Photosynthetic electron transport
• ISSN: 0166-8595; 1573-5079
• DOI: 10.1007/s11120-018-0513-0

Humic substances (HSs) can influence the growth and composition of freshwater phytoplankton assemblage. Since HSs contain many phenolic and quinonic moieties and cause growth reductions in eco-physiological field experiments, HSs are considered photosystem II herbicides. To test this specific mode of action in vivo and in vitro, respectively, we used intact cells of the green alga Desmodesmus armatus , as well as thylakoids isolated from spinach ( Spinacia oleracea ) as a model system for the green algal chloroplast. Photosynthetic electron transport was measured as oxygen evolution and variable chlorophyll fluorescence. The in vivo effect of the artificial humic substance HS1500 on algae consisted of no impact on photosynthesis–irradiance curves of intact green algae compared to untreated controls. In contrast, addition of HS1500 to isolated thylakoids resulted in light-induced oxygen consumption (Mehler reaction) as an in vitro effect. Fluorescence induction kinetics of HS-treated thylakoids revealed a large static quenching effect of HS1500, but no inhibitory effect on electron transport. For the case of intact algal cells, we conclude that the highly hydrophilic and rather large molecules of HS1500 are not taken up in effective quantities and, therefore, cannot interfere with photosynthesis. The in vitro tests show that HS1500 has no inhibitory effect on photosystem II but operates as a weak, oxygen-consuming Hill acceptor at photosystem I. Hence, the results indicate that eco-physiological field experiments should focus more strongly on effects of HSs on extracellular features, such as reducing and red-shifting the underwater light field or influencing nutrient availability by cation exchange within the plankton network.