Rapid detection of FMO3 single nucleotide polymorphisms using a pyrosequencing method

• Published in: Molecular medicine reports Vol. 25; no. 2
• Main Authors: Park, Jin-Woo, Park, In-Hwan, Kim, Jong-Min, Noh, Ji, Kim, Kyoung-Ah, Park, Ji-Young
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.02.2022; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Adult; Asians - genetics; Biotechnology; Dimethylaniline monooxygenase (N-oxide-forming); DNA sequencing; Ethnic groups; Flavin; Gene Frequency; Genetic aspects; Genetic research; Healthy Volunteers; High-Throughput Nucleotide Sequencing - methods; Humans; Linkage analysis; Linkage disequilibrium; Male; Methods; Middle Aged; Minority & ethnic groups; Nucleotide sequencing; Oxidoreductases; Oxygenases - blood; Oxygenases - genetics; Polymorphism, Single Nucleotide; Population; Reproducibility of Results; Republic of Korea; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Software; Young Adult; Republic of Korea; South Korea; c.441C>T; pyrosequencing; c.855C>T; c.472G>A; c.923A>G; flavin‑containing monooxygenase 3
• ISSN: 1791-2997; 1791-3004; 1791-3004
• DOI: 10.3892/mmr.2021.12564

The present study aimed to develop a reliable pyrosequencing method to detect four single nucleotide polymorphisms (SNPs) of the flavin‑containing monooxygenase 3 ( ) gene and to compare the ethnic differences in their allelic frequencies. The pyrosequencing method was used to detect four SNPs, namely, c.855C>T (N285N, rs909530), c.441C>T (S147S, rs1800822), c.923A>G (E308G, rs2266780) and c.472G>A (E158K, rs2266782). The allelic frequencies of these SNPs in 122 unrelated Korean subjects were as follows: i) 44.7% for c.855C>T; ii) 23.4% for c.441C>T; iii) 23.0% for c.923A>G; and iv) 27.1% for c.472G>A. Linkage disequilibrium (LD) analysis revealed that the SNPs c.923A>G and c.472G>A exhibited a strong LD (D'=0.8289, r =0.5332). In conclusion, the pyrosequencing method developed in this study was successfully applied to detect the c.855C>T, c.441C>T, c.923A>G and c.472G>A SNPs of .