Simple high-speed confocal line-scanning microscope

Using a line scan camera and an acousto-optic deflector (AOD), we constructed a high-speed confocal laser line-scanning microscope that can generate confocal images (512 x 512 pixels) with up to 191 frames/s without any mechanically moving parts. The line scanner consists of an AOD and a cylindrical...

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Bibliographic Details
Published inOptics express Vol. 13; no. 13; pp. 5151 - 5156
Main Authors Im, Kang-Bin, Han, Sumin, Park, Hwajoon, Kim, Dongsun, Kim, Beop-Min
Format Journal Article
LanguageEnglish
Published United States 27.06.2005
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Summary:Using a line scan camera and an acousto-optic deflector (AOD), we constructed a high-speed confocal laser line-scanning microscope that can generate confocal images (512 x 512 pixels) with up to 191 frames/s without any mechanically moving parts. The line scanner consists of an AOD and a cylindrical lens, which creates a line focus sweeping over the sample. The measured resolutions in z (depth), x (perpendicular to line focus), and y (direction of line focus) directions are 3.3 mum, 0.7 mum and 0.9 mum, respectively, with a 50x objective lens. This confocal microscope may be useful for analyzing fast phenomena during biological and chemical interactions and for fast 3D image reconstruction.
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ISSN:1094-4087
1094-4087
DOI:10.1364/opex.13.005151