Enzymatic Synthesis of Redox-Labeled RNA and Dual-Potential Detection at DNA-Modified Electrodes

Ferrocene and anthraquinone were used to label RNA (red, dark blue), which could then be detected at an electrode surface bearing complementary DNA oligonucleotide probes (light blue). This approach is an electrochemical analog of popular fluorescence methods for gene expression analysis.

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Bibliographic Details
Published inAngewandte Chemie (International ed.) Vol. 43; no. 21; pp. 2809 - 2812
Main Authors Di Giusto, Daniel A., Wlassoff, Wjatschesslaw A., Giesebrecht, Susanne, Gooding, J. Justin, King, Garry C.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 17.05.2004
WILEY‐VCH Verlag
Subjects
anthraquinones
Biosensing Techniques
DNA - biosynthesis
DNA - chemistry
DNA - genetics
DNA-Directed RNA Polymerases - chemistry
Electrochemistry
Electrodes
ferrocenes
Gene Expression Regulation
Indicators and Reagents
monolayers
Oxidation-Reduction
RNA - biosynthesis
RNA - chemistry
RNA - genetics
RNA polymerase
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Summary:Ferrocene and anthraquinone were used to label RNA (red, dark blue), which could then be detected at an electrode surface bearing complementary DNA oligonucleotide probes (light blue). This approach is an electrochemical analog of popular fluorescence methods for gene expression analysis.
Bibliography:This work was supported by the Australian Research Council (grant LP0347371).
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ArticleID:ANIE200352977
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content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200352977