Fibrinogen Patterns and Activity on Substrates with Tailored Hydroxy Density
The influence of the surface fraction of OH groups on fibrinogen (FG) adsorption is investigated in copolymers of ethyl acrylate and hydroxy ethylacrylate. The amount of adsorbed FG, quantified by western‐blotting combined with image analysis of the corresponding bands, decreases as the hydrophilici...
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Published in | Macromolecular bioscience Vol. 9; no. 8; pp. 766 - 775 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
11.08.2009
WILEY‐VCH Verlag Wiley-VCH |
Subjects | |
Online Access | Get full text |
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Summary: | The influence of the surface fraction of OH groups on fibrinogen (FG) adsorption is investigated in copolymers of ethyl acrylate and hydroxy ethylacrylate. The amount of adsorbed FG, quantified by western‐blotting combined with image analysis of the corresponding bands, decreases as the hydrophilicity of the substrate increases. The influence of substrate wettability on FG conformation and distribution is observed by atomic force microscopy (AFM). The most hydrophobic substrate promotes FG fibrillogenesis, which leads to a fibrin‐like appearance in the absence of any thrombin. The degree of FG interconnection was quantified by calculating the fractal dimension of the adsorbed protein from image analysis of the AFM results. The biological activity of the adsorbed FG is correlated to cell adhesion on FG‐coated substrates.
Fibrinogen adsorption on model substrates with controlled OH density is quantified by western‐blotting and its conformation directly observed by AFM. Substrate‐induced fibrinogen fibrillogenesis is enhanced on some substrates as a consequence of protein material interactions, in absence of either any thrombin or cells. The protein conformation is related to initial cell adhesion. |
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Bibliography: | FEDER ArticleID:MABI200800332 ark:/67375/WNG-T80WFKMJ-3 istex:52FDC1EA84B85328DC0E1BF54EC8DDD3993658EF These authors contributed equally to this work. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1616-5187 1616-5195 |
DOI: | 10.1002/mabi.200800332 |