Chemical defence responses of Norway spruce to two fungal pathogens

• Published in: Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie Vol. 50; no. 6
• Main Authors: Axelsson, Karolin, Zendegi‐Shiraz, Amene, Swedjemark, Gunilla, Borg‐Karlson, Anna‐Karin, Zhao, Tao, Hietala, Ari Mikko
• Format: Journal Article
• Language: English
• Published: Berlin Wiley Subscription Services, Inc 01.12.2020
• Subjects: Bark; Breeding; Chemical defense; Diterpenes; Endoconidiophora polonica; Fungi; Gas chromatography; Genotypes; Heterobasidion parviporum; Hexanes; induced response; Inoculation; Insects; Markers; Mass spectrometry; Mass spectroscopy; Pathogens; Pest resistance; Picea abies; Picea abies clone; Picea abiesclone; Pine trees; Stem rot; Stems; Terpenes; Trees; Norway
• ISSN: 1437-4781; 1439-0329; 1439-0329
• DOI: 10.1111/efp.12640

Constitutive and inducible terpene production is involved in conifer resistance against insects and fungal infestations. To gain knowledge about local defence responses of Norway spruce bark against pathogens and to find potential chemical markers for resistance breeding, we inoculated the stem of 8‐year‐old Norway spruce (Picea abies) clonal trees with both Endoconidiophora polonica (Ep, a common fungal pathogen associated with the spruce bark beetle Ips typographus) and Heterobasidion parviporum (Hp, a severe pathogen causing root and stem rot disease). Three weeks after inoculation, the fungal‐inoculated and intact bark from each tree was sampled. The terpenes in tree bark were extracted with hexane and characterized by gas chromatography–mass spectrometry (GC‐MS). The two fungi induced varied terpene responses in the four spruce clones used. Three of the clones showed a 2.3‐fold to 5.7‐fold stronger terpene response to Hp relative to Ep inoculation, while one clone responded similarly to inoculation with the two fungal pathogens. The amount of the diterpenes thunbergol and geranyllinalool varied between the clones. The level of thunbergol was higher in both intact and fungal‐inoculated bark from the less susceptible clones compared with the more susceptible clones. Geranyllinalool was present in higher amounts in the susceptible clones and is thus a possible marker for susceptibility. Our observations show that Norway spruce employs a similar chemical mechanism against the two fungal pathogens. Based on the present and earlier published data, we suggest that certain Norway spruce genotypes have a strong defence reaction against these two pathogens. The diterpenes thunbergol and geranyllinalool might be useful markers of susceptibility in tree‐breeding programmes and should be the focus of further detailed investigations.