Oxidation stability of yeast biodiesel using Rancimat analysis: validation using infrared spectroscopy and gas chromatography–mass spectrometry

• Published in: Environmental science and pollution research international Vol. 26; no. 3; pp. 3075 - 3090
• Main Authors: Tamilalagan, Anbarasan, Singaram, Jayanthi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2019; Springer Nature B.V
• Subjects: Alternative fuels; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biodegradation; biodiesel; Biodiesel fuels; Biofuels; Chromatography; Degradation; Degradation products; Diesel; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Gas chromatography; gas chromatography-mass spectrometry; Industrial wastes; Infrared analysis; Infrared spectroscopy; Mass spectrometry; Mass spectroscopy; microbial oils; Microorganisms; Oxidation; oxidative stability; Research Article; Shelf life; spectral analysis; Stability analysis; Storage conditions; storage quality; Storage stability; storage time; Waste Water Technology; Water Management; Water Pollution Control; Yeast; yeasts; Induction time; Microbial biodiesel; Thermal oxidative stability; Fourier-transform infrared spectroscopy; Storage stability; Gas chromatography–mass spectrometry
• ISSN: 0944-1344; 1614-7499; 1614-7499
• DOI: 10.1007/s11356-018-3619-1

Biodiesel and single cell oils obtained from oleaginous yeasts grown in industrial waste are attractive alternatives to the conventional fuels. However, there are only few articles dealing with the stability of the microbial biofuels. Hence, this study aimed at characterizing the storage time of biodiesels using Rancimat methods. The microbial oil and the biodiesel obtained from microbial oil have been characterized with storage stability due to various oxidizing and thermal damage. Here, the microbial fuels were subject to Rancimat analysis and found to have high thermal-oxidative stability of 18 and 8.78 h for biodiesel and oil, respectively. The storage stability resulting from storage conditions was extrapolated for biodiesel and oil and has been found to be 1.62 and 0.54 years, respectively. The infrared spectroscopic analysis reveals the degree of oxidation found after the induction time was reached and shows the characteristic peaks for degradation products. Gas chromatography revealed the compounds that were responsible for the stability as well as the amount of degradation products left.